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Silver Nitrate Spray is a silver nitrate gas used by humans to determine if someone is a Lycan. In the case that a Lycan is found, the spray can be used to weaken them, and, in some cases, to kill the Lycan. On contact, the spray burns the skin of the Lycan and, if breathed in, it will burn them internally.

The spray is based on the lethal allergy to silver that all Lycans and Werewolves exhibit. IPU soldiers commonly set up roadblocks and test those trying to escape the city by spraying the drivers with the spray. If the driver is a Lycan, they will quickly become very ill due to the silver contained in the spray.


In the 15-minute anime, Underworld: Endless War. Endless War depicts two IPU agents spraying people caught in a roadblock with the gas to see how they'd react.

In Underworld: Awakening, IPU soldiers are briefly seen spraying a suspected Lycan with it and them transforming and roaring at them as a result.

Silver Nitrate Bombs are shown to contain an explosive version of the spray. When Selene bombed Antigen Headquarters with silver nitrate bombs, the bombs released the spray, killing Lycan guards on every floor where she had planted bombs. Silver nitrate grenades also released the spray, killing a Lycan guard Selene directed the spray towards with a ventilation fan and Quint Lane when Selene stuffed one of the grenades in his stomach.

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